Publications

2020

  • R. Lee*, L. Campanello*, M.J. Hourwitz, A. Omidvar, P. Alvarez, J.T. Fourkas, W. Losert. "Quantifying topography-guided actin dynamics across scales using optical flow." Mol Biol Cell. 2020 Feb 5:mbcE19110614. (Accepted; * indicates equal contribution).
  • Zhao S, Mehta AS, Zhao M. Biomedical Applications of Electrical Stimulation. Cell Mol Life Sci. 2020 Jan 23[Online ahead of print] PMID: 31974658 DOI: 10.1007/s00018-019-03446-1
  • Ferreira F, Luxardi G, Reid B, Ma L, Raghunathan V, Zhao M. “Real-time physiological measurements of oxygen using a non-invasive self-referencing micro-optrode”. Nature Protocols. 2020;15:207-235. PMID: 31925402
  • Luu KY, Zhao M, Mannis MJ. The Use of Electrotherapeutics in Ophthalmology. Am J Ophthalmol. 2020 Mar;211:4-14. doi: 10.1016/j.ajo.2019.11.011. [Epub ahead of print] PMID: 31738895
  • Shen, Y., B. U. S. Schmidt, H. Kubitschke, E. W. Morawetz, B. Wolf, J. A. Käs and W. Losert (2020). "Detecting heterogeneity in and between breast cancer cell lines." Cancer Convergence 4(1): 1.

2019

  • J.T. Fourkas, Wolfgang Losert. "Nanotextured Surfaces for Studying and Controlling Cellular Behavior." Proceedings of International Congress on Applications of Lasers & Electro-Optics (Orlando, October 7-10 2019).
  • L. Guo, H. Li, Y Wang, Z. Li, J. Albeck, M. Zhao, Q. Qing. “Controlling ERK activation dynamics in mammary epithelial cells with alternating electric fields through microelectrodes.” Nano Lett. 2019 Sep 5. Epub ahead of print. https://doi.org/10.1021/acs.nanolett.9b03411
  • K.K. Gokoffski, et al., "Physiologic Electrical Fields Direct Retinal Ganglion 1 Cell Axon Growth In Vitro.” IOVS. 2019. 2019;60:3659–3668. https://doi.org/10.1167/iovs.18-25118.
  • E.A. Lescak*, K.M. O'Neill*, et al., "Ten simple rules for providing a meaningful research experience to high-school students," PLOS Computational Biology, 2019.
  • K. Zhu et al., “Expression of integrins to control migration direction of electrotaxis,” The FASEB Journal, p. fj.201802657R, 2019.
  • Y. Sun et al., “Infection-generated electric field in gut epithelium drives bidirectional migration of macrophages,” PLOS Biology, vol. 17, no. 4, pp. 1–29, 2019.
  • E. A. Lescak, K. M. O’Neill, G. M. Collu, and S. Das, “Ten simple rules for providing a meaningful research experience to high school students,” PLOS Computational Biology, vol. 15, no. 4, pp. 1–7, 2019.
  • Y. Miao et al., “Wave patterns organize cellular protrusions and control cortical dynamics,” Molecular Systems Biology, vol. 15, no. 3, p. e8585, 2019.

2018

  • A. Li et al., “Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks,” Nature Communications, vol. 9, no. 1, p. 5377, Dec. 2018.
  • F. Ferreira, V. Raghunathan, G. Luxardi, K. Zhu, and M. Zhao, “Early redox activities modulate Xenopus tail regeneration,” Nature Communications, vol. 9, no. 1, p. 4296, Oct. 2018.
  • M. Edwards, H. Cai, B. Abubaker-Sharif, Y. Long, T. J. Lampert, and P. N. Devreotes, “Insight from the maximal activation of the signal transduction excitable network in Dictyostelium discoideum,” Proceedings of the National Academy of Sciences, vol. 115, no. 16, pp. E3722–E3730, 2018.
  • O. Ilina et al., “Intravital microscopy of collective invasion plasticity in breast cancer,” Disease Models & Mechanisms, vol. 11, no. 9, 2018.
  • Y. Shen, B. Chaigne-Delalande, R. Lee, and W. Losert, “CytoBinning: immunological insights from multi-dimensional data.,” BioRxiv, vol. 321893, 2018.
  • Y. Shen, N. Kubben, J. Candia, A. V Morozov, T. Misteli, and W. Losert, “RefCell: Multi-dimensional analysis of image-based high-throughput screens based on ‘typical cells,’” BioRxiv, vol. 325415, 2018.
  • X. Sun, M. J. Hourwitz, E. M. Baker, B. U. S. Schmidt, W. Losert, and J. T. Fourkas, “Replication of biocompatible, nanotopographic surfaces,” Scientific Reports, vol. 8, no. 1, 2018.
  • Y. H. Sun et al., “Electric fields accelerate cell polarization and bypass myosin action in motility initiation,” Journal of Cellular Physiology, vol. 233, no. 3, pp. 2378–2385, 2018.
  • C. H. Stuelten, R. M. Lee, W. Losert, and C. A. Parent, “Lysophosphatidic acid regulates the motility of MCF10CA1a breast cancer cell sheets via two opposing signaling pathways,” Cellular Signalling, vol. 45, pp. 1–11, 2018.
  • X. Sun et al., “Src activation decouples cell division orientation from cell geometry in mammalian cells,” Biomaterials, vol. 170, pp. 82–94, 2018.

2017

  • T. J. Lampert et al., “Shear force-based genetic screen reveals negative regulators of cell adhesion and protrusive activity,” Proceedings of the National Academy of Sciences, vol. 114, no. 37, pp. E7727--E7736, 2017.
  • L. Li et al., “Caveolin-1-mediated STAT3 activation determines electrotaxis of human lung cancer cells,” Oncotarget, vol. 8, no. 56, p. 95741, 2017.
  • Y. Miao et al., “Altering the threshold of an excitable signal transduction network changes cell migratory modes,” Nature cell biology, vol. 19, no. 4, p. 329, 2017.
  • X. Jiao, Y. Wang, and Q. Qing, “Scalable Fabrication Framework of Implantable Ultrathin and Flexible Probes with Biodegradable Sacrificial Layers,” Nano letters, vol. 17, no. 12, pp. 7315–7322, 2017.
  • Y. Zhang et al., “Collective cell migration has distinct directionality and speed dynamics,” Cellular and Molecular Life Sciences, vol. 74, no. 20, pp. 3841–3850, 2017.
  • P. N. Devreotes, S. Bhattacharya, M. Edwards, P. A. Iglesias, T. Lampert, and Y. Miao, “Excitable signal transduction networks in directed cell migration,” Annual review of cell and developmental biology, vol. 33, pp. 103–125, 2017.

2016

  • Y. Shen et al., “Diabetic cornea wounds produce significantly weaker electric signals that may contribute to impaired healing,” Scientific Reports, vol. 6, p. 26525, 2016.
  • Y. Artemenko, L. Axiotakis, J. Borleis, P. A. Iglesias, and P. N. Devreotes, “Chemical and mechanical stimuli act on common signal transduction and cytoskeletal networks,” Proceedings of the National Academy of Sciences, vol. 113, no. 47, pp. E7500--E7509, 2016.
  • F. Ferreira, G. Luxardi, B. Reid, and M. Zhao, “Early bioelectric activities mediate redox-modulated regeneration,” Development, p. dev--142034, 2016.
  • Y.-H. Sun et al., “An Experimental Model for Simultaneous Study of Migration of Cell Fragments, Single Cells, and Cell Sheets,” in Chemotaxis: Methods and Protocols, T. Jin and D. Hereld, Eds. New York, NY: Springer New York, 2016, pp. 251–272.
  • K. Nakajima and M. Zhao, “Concerted action of KCNJ15/Kir4. 2 and intracellular polyamines in sensing physiological electric fields for galvanotaxis,” Channels, vol. 10, no. 4, pp. 264–266, 2016.

Book Chapters

  • Zhao M., Baidya S., and Hassan A., “Computational Modeling of Transepithelial Endogenous Electric Signals,” in Handbook of Biological Effects of Electromagnetic Fields (Vol 1, 4th Edition), G. B. and B. F., Eds. CRC Press/Taylor and Francis Group, 2018.
  • Zhao M., “Bioelectrics – an underappreciated and fertile field in biology,” in Ahead of the Curve: Hidden breakthroughs in the biosciences: Volume 2., M. Levin and D. Adams., Eds. Institute of Physics Publishing, 2018. ISBN-13: 978-0750313278 ISBN-10: 0750313277

Patents

  • Q. Qing, C.W. Tsao, P. Zhang. “Apparatus and methods for synthesizing biopolymers.” International (PCT) patent application no. PCT/US19/4026, filed July 2019.

Presentations

  • K.M. O’Neill, E.K. Robinson, W. Losert. “Spatiotemporal dynamics of the neuronal cytoskeleton across scales during development.” American Physical Society’s Annual March Meeting (Oral Presentation, Physics of the Cytoskeleton Across Scales IV: In vivo, Denver, CO). March 6, 2020. [conference cancelled due to COVID-19]
  • Q. Yang, M. Hourwitz, L. Campanello, B. Shariff, P. Devreotes, J.T. Fourkas, W. Losert. “Electric Field Guidance of Actin Waves.” American Physical Society’s Annual March Meeting (Oral Presentation, Physics of the Cytoskeleton Across Scales I: Mechanics and Rheology, Denver, CO). March 3, 2020. [conference cancelled due to COVID-19]
  • L. Campanello, M. Traver, B. Schaefer, W. Losert. “Analysis and simulations of Bcl10 self-assembly and degradation in activated T cells.” American Physical Society’s Annual March Meeting (Oral Presentation, Cellular Biophysics: structure, mechanics, and dynamics, Denver, CO). March 2, 2020. [conference cancelled due to COVID-19]
  • R.M. Lee, M.I. Vitolo, W. Losert, S.S. Martin. “Distinct Roles of Tumor-Associated Mutations in Collective Cell Migration.” American Physical Society’s Annual March Meeting (Oral Presentation, Mechanics of Cells and Tissues Across Scales I, Denver, CO). March 2, 2020. [conference cancelled due to COVID-19]
  • Losert, Wolfgang, Quantum Biology, "Cell Dynamics and Excitable Systems." (February 13, 2020).
  • Losert, Wolfgang, "Understanding and Controlling Excitable Networks in Living Systems," Air Force Research Laboratory. (January 22, 2020).
  • Losert, Wolfgang, "Physical Guidance of Cells," Johns Hopkins University. (December 5, 2019).
  • S. Mukherjee, K.M. O’Neill, B.L. Firestein, W. Losert, B. Babadi. “A statistical approach to the dynamic analysis of synchronous spiking in neuronal ensembles.” Annual Meeting of the Society for Neuroscience (Poster Presentation 707.19, Chicago, IL). October 23, 2019.
  • K.M. O’Neill*, E. Saracino*, L. Maiolo, M.G. Mola, A. Convertino, V. Guarino, T. Posati, M. Caprini, G. Fortunato, R. Zamboni, L. Ambrosio, G.P. Nicchia, W. Losert, V. Benfenati (2019). “Impact of actin waves on ion and water membrane dynamics in astrocytes.” Annual Meeting of the Society for Neuroscience (Poster Presentation 204.06, Chicago, IL). October 20, 2019. (*equal contribution).
  • K.M. O’Neill, W. Losert (2019). “Using extracellular cues to bias the intracellular dynamics of neural cells.” Annual Meeting of the Biomedical Engineering Society (Oral Presentation, Materials and Devices to Drive and Record the Behavior of Neural Cells I, Philadelphia, PA). October 18, 2019.
  • J. Fourkas. Keynote. “Nanotextured Surfaces for Studying and Controlling Cellular Behavior,” ICALEO 2019, Orlando, FL, October 7-10, 2019.
  • Losert, Wolfgang, Nonclassical Biophysics, "Collective Behavior," AFOSR. (September 22, 2019).
  • E. Saracino, A.l. Borrachero-Conejo, L. Maiolo, V. Guarino, K.M. O’Neill, D. Polese, F. Formaggio, M. Caprini, M. Muccini, G. Fortunato, L. Ambrosio, R. Zamboni, W. Losert, A. Convertino, V. Benfenati (2019). “Nanostructured Interfaces Enable in Vivo-Like Differentiation of Primary Astrocytes and Allow Multiscale Study of their Functionality in Vitro.” XIV European Meeting on Glial Cells in Health and Disease (Poster Presentation, Porto, Portugal). July 11, 2019.
  • Losert, Wolfgang, International Physics of Living Systems Symposium, "Physical Guidance of Cells," PoLS. (July 2019).
  • M. Zhao, B. Reid, G. Luxardi, F. Ferreira. “Electric fields/currents at wounds: The biological significance, their origin and nature.” Session on Endogenous Electricity in Biology. PIERS 41st Meeting (Rome, Italy). June 20, 2019.
  • Losert, Wolfgang, Packing Meeting, "Collective Dynamics," Yale University. (June 6, 2019).
  • J. Fourkas. “Nanotopographic Control of Cytoskeletal Dynamics,” Department of Chemistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil, June 3, 2019.
  • Q. Qing. Talk “A New Way to Modulate Protein Dynamics in Live Cells: Precisely Regulate ERK Signaling Pathway with Local Electric Fields.” CML Symposium at Harvard University, April 20, 2019.
  • A. Contreras, K. Zhu, R. Lee, Y. Zhang, L. Guo, L. Campanello, W. Losert, M. Zhao. “Direct Current Electric Fields Induce Collective Migration of Human Corneal Epithelial Monolayers.” Biochemistry and Molecular Biology. Experimental Biology 2019 in Orlando. Topic Category: 213-ASBMB Interdisciplinary/Translational Science (SEBM). April 6-9, 2019.
  • M. Hu, M. Darrow, H. Li, Y. Wang, Q. Qing. “Topographical regulation of migration and actin propagation in Dictyostelium discoideum.” “Tracking Protein Dynamics by Single Molecule Conductance Measurements with Fixed-Nanogap Devices”. “Precise ERK activation by local AC electric field.” Posters and talks. Biophest. March 30, 2019.
  • K.K. Gokoffski, on the abstract submission entitled "Guidance of Retinal Ganglion Cell Axon Growth Using Electrical Fields for Optic Nerve Regeneration" for the American Glaucoma Society 29th Annual Meeting, San Francisco, CA. March 14-17, 2019.
  • S.J. Gates, K.M. O’Neill, P. Alvarez, S. Aghayee, W. Losert. Contributed talk. “Propagation of Electrical Activity Coupled to Actin Dynamics in Electrically Active Neuron-Like Cells.” American Physical Society’s Annual March Meeting (Oral Presentation, Controlling Cells with Electric Fields, Boston, MA). March 5, 2019.
  • Q. Yang, M.J. Hourwitz, L. Campanello, B. Sahrif, P.N. Devreotes, J.T. Fourkas, W. Losert. Contributed talk. “Large-scale Actin Wave Patterns Perturbed by Electric and Mechanical Cues in Giant Dictyostelium discoideum.” American Physical Society’s Annual March Meeting (Oral Presentation, Controlling Cells with Electric Fields, Boston, MA). March 5, 2019.
  • M.J. Hourwitz, J.T. Fourkas, W. Losert. Contributed talk. “Tuning Migratory and Cytoskeletal Response of Cells to Texture with Collagen-IV Coating.” American Physical Society’s Annual March Meeting (Oral Presentation, Physics of the Cytoskeleton Across Scales III, Boston, MA). March 5, 2019.
  • P. Alvarez, S.J. Gates, S. Aghayee, K.M. O’Neill, G. Frank, W. Losert. Contributed talk. "Investigating Wave Morphodynamics in Electrically Active Cell Sheets." American Physical Society’s Annual March Meeting (Oral Presentation, Controlling Cells with Electric Fields, Boston, MA). March 5, 2019.
  • K.M. O’Neill, W. Losert. Invited. “Controlling the Intracellular Dynamics of Neuronal Model Systems.” American Physical Society’s Annual March Meeting (Oral Presentation, Controlling Cells with Electric Fields, Boston, MA). March 5, 2019.
  • Q. Qing. Invited. “Precisely Regulate ERK Signaling Pathway with Local Electric Fields.” American Physical Society’s Annual March Meeting (Oral Presentation, Controlling Cells with Electric Fields, Boston, MA). March 5, 2019
  • Bull A. M.J. Hourwitz, L. Campanello, J.T. Fourkas, W. Losert. Contributed talk. “Control of Immune Cells via Chemical, Electrical and Mechanical Cues.” American Physical Society’s Annual March Meeting (Oral Presentation, Controlling Cells with Electric Fields, Boston, MA). March 5, 2019.
  • M. Zhao. Invited presentation. “Electrical Stimulation for Wound Management – Is There a Scientific Basis?” (Brief Introduction and some laboratory insights). Center for Health and Technology. Sacramento, CA. February 14, 2019.
  • Q. Liu, A. Contreras. “Transcranial Electrical Stimulation Activates Endogenous Neural Stem Cells in Alzheimer’s Disease Brain.” UC Davis, Alzheimer’s Disease Center. February 7, 2019.
  • P.N. Devreotes, Physiology, Biophysics and Systems Biology (PBSB) Seminar Series, Weill Cornell Medicine, New York, NY. 2019.
  • P.N. Devreotes, Gordon Research Conference on “Directed Cell Migration.” Galveston, TX. 2019.
  • P.N. Devreotes, Imaging Science and Cancer Biology Symposium, Hood College, Frederick National Laboratory for Cancer Research, Frederick, MD. 2019.
  • Q. Qing. Invited talk. “Precise Modulation of ERK Signaling Pathway by Electric Field.” Biophysics Seminar at University of Maryland, 2019.
  • M. Zhao. Invited Seminar. “Intracellular Signaling Regulated by Electric Fields.” Frank Reidy Research Center for Bioelectrics at Old Dominion University. Norfolk, Virginia. December 4, 2018.
  • W. Losert, K. O’Neill. “Decoding the Natural Mechanical Rhythms of Brain Cells.” 13th US-Italy Joint Commission Meeting on Science and Technology Cooperation: Advanced Materials and Nanotechnologies Working Group. December 3, 2018.
  • S.J. Gates, P. Alvarez, R. Lee, M.J. Hourwitz, J.T. Fourkas, W. Losert. “Differential Gen Expression in Response to Esotaxis Cues.” American Society for Cell Biology & European Molecular Biology 2018. Poster Session: Signaling Networks Governing Cell Migration Poster Presentation 11652). December 2018.
  • Q. Qing. Talk. “Precise Control of ERK Activation in Epithelial Cells by Local AC Electric Fields.” MRS Fall Meeting. November 25-30, 2018.
  • M. Zhao. Invited Seminar. “The Effects of Electrical Stimulation on the Tissues and the Cells of the Ocular Surface and Adnexa.” Pleasanton, CA 94588. coopervision.com. November 19 2018.
  • M. Zhao. Invited Seminar. “Biology and Electricity.” Celebration of 80 Anniversary of National Southwestern Associated University. Kunming, China. November 1, 2018.
  • K. Zhu and A. Contreras. “Electrically Regulating Activation Dynamics of Intracellular Signaling Pathways.” EPI-BIO meeting, UC Davis, Department of Dermatology. October 24, 2018.
  • K.M. O’Neill, S.J. Gates, P.H. Alvarez, Y. Wang, H. Li, Q. Qing, G. Ortiz, E. Miller, S.D. Brenowitz, W. Losert. “Investigating the Internal Responses of Neurons and Neuron-Like Cells to External Electric Fields.” Annual Meeting of the Biomedical Engineering Society (Poster Presentation 824, Atlanta, GA). October 18, 2018.
  • K.M. O’Neill, L.J. Campanello, J. Corbett-Frank, H.Y. Fang, A.Y. Clarke, W. Losert, E. Giniger. Invited. “Robust, Automated Quantification of Actin Dynamics in the Growth Cone of Drosophila Neurons.” Drosophila Neuroscience Colloquium (Oral Presentation, National Institutes of Health, Bethesda, MD). October 12, 2018.
  • K.K. Gokoffski, X. Jia, M. Zhao. “Electrical Fields Direct Retina Ganglion Cell Neurite Growth.” ARVO. 2018.
  • Q. Qing. Invited talk. “Precise Modulation of ERK Signaling Pathway by Electric Field.” Biomedical Engineering Seminar. Tufts University, Boston. October 2018.
  • P.N. Devreotes. Invited Speaker. FASEB Science Research Conference on “Phospholipids: Dynamic Lipid Signaling in Health and Disease.” Steamboat Springs, CO, USA. July 2018.
  • L. Ma, A. Guichard, J. Li, O. Qin, M. Zhao, W. Liu, Y. Tan. “Disturbed Sebum and Microbiome Composition in Sensitive Scalp.” J. Investigative Dermatology. 138 (5): S221-S221 Meeting Abstract: 1302. Meeting Abstract. International Investigative Dermatology (IID) Meeting. Location: Orlando, FL. May 16-19, 2018.
  • Zhao M. Cell and Developmental Program. University College London, Invited Seminar, London, England, April 2017.
  • Li H, Qing Q. Activation of MAPK/ERK in epithelial cells by localized AC electric field. Biophest, Invited Seminar, University of Arizona, USA, April 19, 2017
  • O’Neill KM, Firestein BL. Brain-Derived Neurotrophic Factor regulates the development of electrical activity in hippocampal neuron networks cultured on microelectrode arrays. Annual Meeting of the Society for Neuroscience, Oral Presentation, Control of Neuronal Firing 542.04, Washington, D.C., Nov 14, 2017. (Losert W, MURI grant cited as support)
  • Losert W, Aghayee S, Winkowski D, Kanold P. Particle Tracking based Motion Compensation for neuronal imaging. Annual Meeting of the Society for Neuroscience, Poster Presentation, Washington, DC, November 15, 2017
  • Zhao M. Electrically guiding human neural stem cells in the brain -- implications in neurological diseases (e.g. stroke). 2017 Zhongshan International Neurology Summit Conference, Plenary Talk, Guangzhou, China, Nov 25-26, 2017.
  • Zhao M. Electrical Signaling as a Novel and Powerful Mechanism in Wound Healing and Regeneration -- an exciting opportunity in regenerative medicine. Zhongshan Ophthalmic Center, Sun Yat-sen University (ZOC), Invited Seminar, Guangzhou, China, Nov 28, 2017.
  • Qing Q. Nanopore device with self-aligned nanogap electrodes for DNA characterization (Invited Talk), and Reconcile rigidity and flexibility for implantable probes (Poster). International Conference on Advances in Functional Materials, University of California, Los Angeles Campus, USA, Aug 14, 2017.
  • Ferreira F, Luxardi G, Raghunathan V, Reid B, Zhao M. A decade integrating biology (in) research and training. 10 years of the Master in Evolutionary and Developmental Biology (BED), Faculdade de Ciências da Universidade de Lisboa, Portugal, Dec 21, 2017.
  • Losert W, Invited Seminar, Old Dominion University, January 2018
  • Bull A, Hourwitz MJ, Campanello L, Omidvar A, Schmidt S, Fourkas JT, Losert W. Physical Control of Actin Wave Dynamics and Migration of Neutrophil-like HL60 Cells. American Physical Society March Meeting, Oral Presentation, Biophysics of Cellular Organization and Dynamics Across Multiple Spatial Scales B49.00005, Los Angeles, CA, March 5, 2018.
  • Alvarez P, Lee RM, Hourwitz MJ, Campanello L, Camp C, Cicerone M, Fourakas JT, Losert W. Using Topographical Guidance to Investigate Cytoskeletal Excitability. American Physical Society March Meeting, Oral Presentation, Biophysics of Cellular Organization and Dynamics Across Multiple Spatial Scales B49.00012, Los Angeles, CA, March 5, 2018.
  • Schmidt S, Hourwitz MJ, Fourkas J, Losert W. Influence of Electrotaxis, Chemotaxis and Textured Surfaces on Actin Dynamics. American Physical Society March Meeting, Oral Presentation, Biophysics of Cellular Organization and Dynamics Across Multiple Spatial Scales V51.00009, Los Angeles, CA, March 8, 2018.
  • Aghayee S, Winkowski D, Kanold P, Losert W. Reliable targeting method for in vivo photo-stimulation of neurons. American Physical Society March Meeting, Oral Presentation, Neural Control of Behavior S06.00009, Los Angeles, CA, March 8, 2018.
  • Lee RM, Hourwitz MJ, Thompson K, Vitolo M, Fourkas JT, Losert W, Martin SS. Response of collective cell migration to surface topography reveals distinct phenotypes. American Physical Society March Meeting, Biophysics of Cellular Organization and Dynamics Across Multiple Spatial Scales V51.00004, Oral Presentation, March 8, 2018